Journal: Journal of Dental Sciences
Article Title: Flammulina velutipes polysaccharides exhibit potent antioxidant and anti-pyroptotic properties in diabetes-associated periodontitis: A preliminary in vitro study
doi: 10.1016/j.jds.2025.07.017
Figure Lengend Snippet: Flammulina velutipes polysaccharides (FVP) attenuated cell senescence triggered by advanced glycation end products (AGEs) and lipopolysaccharide (LPS)-treated human gingival fibroblasts (HGFs). Senescence-associated β-galactosidase (SA-β-gal) activity was measured in HGFs pretreated with AGEs and LPS, followed by the addition of FVP (A). The protein level of senescence marker p16 was determined by Western blot under the same treatment conditions (B).
Article Snippet: Primary antibodies against cell senescence marker p16 and pyroptosis markers ASC (Cell Signaling Inc., Danvers, MA, USA), NLRP3 (Invitrogen Life Technologies, Carlsbad, CA, USA), pro-caspase-1 and cleaved caspase-1 (Abcam, Cambridge, UK), pro-GSDMD and cleaved GSDMD (Cell signaling), pro-IL-1β and IL-1β (Cell signaling), and GAPDH (Invitrogen) were used.
Techniques: Activity Assay, Marker, Western Blot